IFR 128 BioSciences Gerland - Lyon Sud
HCL - bioMérieux
Responsable d'équipe : MALLET François
Cancer Biomarkers Research Group
Adresse :
 
Centre Hospitalier Lyon Sud,
Téléphone :
 33 (0)4 72 67 87 85

Bâtiment 3F,
Fax :
 33 (0)4 72 67 87 87
69495 Pierre Bénite cedex
E-mail :
 Francois.MALLET@eu.biomerieux.com
France
site web :
Thématique de recherche :

Notre recherche vise à comprendre la signification biologique des rétrovirus endogènes humains. Nous avons découvert la famille HERV-W dont l'expression varie quantitativement et qualitativement en fonction des contextes, physiologiques ou pathologiques. Cette famille contient en outre un locus codant pour une enveloppe impliquée dans la morphogénèse placentaire. La nature rétrovirale et cellulaire de ces éléments, leur abondance dans le génome, implique des approches virologiques, génétiques, cellulaires, immunologiques et bioinformatiques telles que développées dans l'IFR.
Principales publications :

Gimenez J, Montgiraud C, Pichon JP, Bonnaud B, Arsac M, Ruel K, Bouton O, Mallet F.
Custom human endogenous retroviruses dedicated microarray identifies self-induced HERV-W family elements reactivated in testicular cancer upon methylation control.
Nucleic Acids Res. 2010 Jan 7.

Gimenez J, Montgiraud C, Oriol G, Pichon JP, Ruel K, Tsatsaris V, Gerbaud P, Frendo JL, Evain-Brion D, Mallet F.
Comparative methylation of ERVWE1/syncytin-1 and other human endogenous retrovirus LTRs in placenta tissues.
DNA Res. 2009 Aug;16(4):195-211. Epub 2009 Jun 27.
Gimenez J, Mallet F .
ERVWE1 (Endogenous Retroviral family W, Env(C7), member 1).
Atlas Genet Cytogenet Oncol Haematol. September 2007
Antony JM, Ellestad KK, Hammond R, Imaizumi K, Mallet F, Warren KG, Power C.
The human endogenous retrovirus envelope glycoprotein, syncytin-1, regulates neuroinflammation and its receptor expression in multiple sclerosis: a role for endoplasmic reticulum chaperones in astrocytes.
J Immunol. 2007 Jul 15;179(2):1210-24
Pichon JP, Bonnaud B, Mallet F.
Quantitative multiplex degenerate PCR for human endogenous retrovirus expression
profiling.
Nat Protoc. 2006;1(6):2831-8.
Drewlo S, Leyting S, Kokozidou M, Mallet F, Potgens AJ.
C-Terminal truncations of syncytin-1 (ERVWE1 envelope) that increase its fusogenicity.
Biol Chem 2006.;387(8):1113-20.
Nellaker C, Yao Y, Jones-Brando L, Mallet F, Yolken RH, Karlsson H.
Transactivation of elements in the human endogenous retrovirus W family by viral infection.
Retrovirology.2006; 6;3:44.
Cheynet V, Oriol G, Mallet F. (2006).
Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein. Retrovirology, 2006; 3:41.
Pichon JP, Bonnaud B, Cleuziat P, Mallet F.
Multiplex degenerate PCR coupled with an oligo sorbent array for human endogenous retrovirus expression profiling.
Nucleic Acids Res. 2006 Mar 22;34(6)
Bonnaud B, Beliaeff J, Bouton O, Oriol G, Duret L, Mallet F.
Natural history of the ERVWE1 endogenous retroviral locus.
Retrovirology. 2005 Sep 22;2:57.
Prudhomme S, Bonnaud B, Mallet F.
Endogenous retroviruses and animal reproduction.
Cytogenet Genome Res. 2005;110(1-4):353-64. Review.
Cheynet V, Ruggieri A, Oriol G, Blond JL, Boson B, Vachot L, Verrier B, Cosset FL, Mallet F.
Synthesis, assembly, and processing of the Env ERVWE1/syncytin human endogenous retroviral envelope.
J Virol. 2005 May;79(9):5585-93.
Prudhomme S, Oriol G, Mallet F.
A retroviral promoter and a cellular enhancer define a bipartite element which controls env ERVWE1 placental expression.
J Virol. 2004 Nov;78(22):12157-68.
Antony JM, van Marle G, Opii W, Butterfield DA, Mallet F, Yong VW, Wallace JL, Deacon RM, Warren K, Power C.
Human endogenous retrovirus glycoprotein-mediated induction of redox reactants causes oligodendrocyte death and demyelination.
Nat Neurosci. 2004 Oct;7(10):1088-95. Epub 2004 Sep 26.
Bonnaud B, Bouton O, Oriol G, Cheynet V, Duret L, Mallet F.
Evidence of selection on the domesticated ERVWE1 env retroviral element involved in placentation.
Mol Biol Evol. 2004 Oct;21(10):1895-901. Epub 2004 Jul 14.
Mallet F, Bouton O, Prudhomme S, Cheynet V, Oriol G, Bonnaud B, Lucotte G, Duret L, Mandrand B.
The endogenous retroviral locus ERVWE1 is a bona fide gene involved in hominoid placental physiology.
Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1731-6. Epub 2004 Feb 02.
Frendo JL, Olivier D, Cheynet V, Blond JL, Bouton O, Vidaud M, Rabreau M, Evain-Brion D, Mallet F.
Direct involvement of HERV-W Env glycoprotein in human trophoblast cell fusion and differentiation.
Mol Cell Biol. 2003 May;23(10):3566-74.
Blond JL, Lavillette D, Cheynet V, Bouton O, Oriol G, Chapel-Fernandes S, Mandrand B, Mallet F, Cosset FL.
An envelope glycoprotein of the human endogenous retrovirus HERV-W is expressed in the human placenta and fuses cells expressing the type D mammalian retrovirus receptor.
J Virol. 2000 Apr;74(7):3321-9.
Blond J.L. , F. Besème, L. Duret,O. Bouton, F. Bedin, H. Perron, B. Mandrand and F. Mallet.
Molecular characterization and placental expression of HERV-W, a new human endogenous retrovirus family.
J. Virol., 1999, 73(2):1175-1185

 

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